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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20902 | 5' | -55.7 | NC_004689.1 | + | 53417 | 0.72 | 0.455477 |
Target: 5'- gGGAGAcUguGgCugAaUCUCCCCCGGu -3' miRNA: 3'- -CCUCUaAguCgGugUcAGAGGGGGCC- -5' |
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20902 | 5' | -55.7 | NC_004689.1 | + | 51178 | 0.69 | 0.609099 |
Target: 5'- aGGuGAUUCGGCCAugauuCAGUCcUCCaagUCGGg -3' miRNA: 3'- -CCuCUAAGUCGGU-----GUCAG-AGGg--GGCC- -5' |
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20902 | 5' | -55.7 | NC_004689.1 | + | 59271 | 0.68 | 0.65177 |
Target: 5'- ------aUAGUCACGGUCUCCCCUcauGGa -3' miRNA: 3'- ccucuaaGUCGGUGUCAGAGGGGG---CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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