Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20905 | 3' | -52.7 | NC_004689.1 | + | 42535 | 0.66 | 0.899828 |
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Target: 5'- gGCgAugGUGCCUCCC-----GAAUGGCa -3' miRNA: 3'- -CG-UugUACGGAGGGgccagUUUACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 61299 | 0.67 | 0.84519 |
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Target: 5'- aCGACAuUGCCaaaCCCCcaucgGGUCAAucgcucgucGUGGCa -3' miRNA: 3'- cGUUGU-ACGGa--GGGG-----CCAGUU---------UACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 42185 | 0.68 | 0.799314 |
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Target: 5'- uGCAGCAUGUCUUggaCCGGaUCGGccugcgcUGGCg -3' miRNA: 3'- -CGUUGUACGGAGg--GGCC-AGUUu------ACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 61253 | 0.7 | 0.695847 |
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Target: 5'- uGCGuuuCggGUCUCCCCGGUUGAucaGGUa -3' miRNA: 3'- -CGUu--GuaCGGAGGGGCCAGUUua-CCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 50165 | 0.72 | 0.58608 |
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Target: 5'- uGUAGC-UGCCgCCCUGGUCGuccuUGGUc -3' miRNA: 3'- -CGUUGuACGGaGGGGCCAGUuu--ACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 9107 | 0.73 | 0.510904 |
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Target: 5'- uCGACAUGCCugUCCCCuacGGgaaUCAGGUGGUu -3' miRNA: 3'- cGUUGUACGG--AGGGG---CC---AGUUUACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 17219 | 0.73 | 0.496322 |
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Target: 5'- cGCcGCGUGCgCUCCCCGGUagccccgucuUGGUu -3' miRNA: 3'- -CGuUGUACG-GAGGGGCCAguuu------ACCG- -5' |
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| 20905 | 3' | -52.7 | NC_004689.1 | + | 29234 | 0.75 | 0.402136 |
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Target: 5'- uCAACGUGCCcaCUCCGGUacgaaucgCAGAUGGCc -3' miRNA: 3'- cGUUGUACGGa-GGGGCCA--------GUUUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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