Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20906 | 3' | -54.9 | NC_004689.1 | + | 41860 | 0.66 | 0.784679 |
Target: 5'- uUCCACGCgGCACG-GUCGGccuuGCUg -3' miRNA: 3'- uAGGUGCGaCGUGCaCGGCUucu-UGA- -5' |
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20906 | 3' | -54.9 | NC_004689.1 | + | 32333 | 0.66 | 0.754361 |
Target: 5'- uUUCACuGCUGguCGgccaGCCGAGGAAUc -3' miRNA: 3'- uAGGUG-CGACguGCa---CGGCUUCUUGa -5' |
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20906 | 3' | -54.9 | NC_004689.1 | + | 22161 | 0.69 | 0.602979 |
Target: 5'- gGUCCACugucgGgaGCACGuUGCCGguGAACg -3' miRNA: 3'- -UAGGUG-----CgaCGUGC-ACGGCuuCUUGa -5' |
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20906 | 3' | -54.9 | NC_004689.1 | + | 10613 | 0.71 | 0.495937 |
Target: 5'- gGUCUGCGgaGCGCcUGCCGGAGAcguuGCg -3' miRNA: 3'- -UAGGUGCgaCGUGcACGGCUUCU----UGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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