Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20907 | 3' | -52.4 | NC_004689.1 | + | 59244 | 0.66 | 0.894596 |
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Target: 5'- cGGUCccUGCUcGCGGCACCA-GuCGCGa -3' miRNA: 3'- -UCAGucACGA-UGCUGUGGUuCuGCGU- -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 32590 | 0.66 | 0.871915 |
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Target: 5'- cGUCGGUGUcagUGAUACCGAGACc-- -3' miRNA: 3'- uCAGUCACGau-GCUGUGGUUCUGcgu -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 58692 | 0.66 | 0.871915 |
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Target: 5'- cGGUcCAGUcGCccuUACGAgaGCCGGGACGCc -3' miRNA: 3'- -UCA-GUCA-CG---AUGCUg-UGGUUCUGCGu -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 7898 | 0.67 | 0.855518 |
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Target: 5'- --aCGGUGCgacgACGACGCUuacGCGCAg -3' miRNA: 3'- ucaGUCACGa---UGCUGUGGuucUGCGU- -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 45703 | 0.67 | 0.838169 |
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Target: 5'- cAGUCuugGGUGCUAC--CACCAGGAuUGCGc -3' miRNA: 3'- -UCAG---UCACGAUGcuGUGGUUCU-GCGU- -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 39158 | 0.68 | 0.810515 |
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Target: 5'- gGGUCAGUG-UGCGGauCACCuucgcuGGCGCGc -3' miRNA: 3'- -UCAGUCACgAUGCU--GUGGuu----CUGCGU- -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 52183 | 0.7 | 0.707656 |
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Target: 5'- cGGUCGGaucggGCUGCGugACUuccucGACGCGc -3' miRNA: 3'- -UCAGUCa----CGAUGCugUGGuu---CUGCGU- -5' |
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| 20907 | 3' | -52.4 | NC_004689.1 | + | 42893 | 0.71 | 0.607135 |
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Target: 5'- -aUCAGUGCaGCGAaugccucCACCAGGACgGCGu -3' miRNA: 3'- ucAGUCACGaUGCU-------GUGGUUCUG-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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