Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20908 | 3' | -55.9 | NC_004689.1 | + | 15738 | 0.66 | 0.70245 |
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Target: 5'- gUCGC-CUGCcAGCGgUGCCUugGg- -3' miRNA: 3'- aAGCGuGACGuUCGUgACGGGugUgu -5' |
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| 20908 | 3' | -55.9 | NC_004689.1 | + | 31230 | 0.66 | 0.690454 |
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Target: 5'- uUUCGCACcgcguaaUGCGAuGCAguCUGCCC-CGCu -3' miRNA: 3'- -AAGCGUG-------ACGUU-CGU--GACGGGuGUGu -5' |
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| 20908 | 3' | -55.9 | NC_004689.1 | + | 38703 | 0.67 | 0.603157 |
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Target: 5'- aUgGCAaUGCGAGCACUGuuCCCACuCGu -3' miRNA: 3'- aAgCGUgACGUUCGUGAC--GGGUGuGU- -5' |
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| 20908 | 3' | -55.9 | NC_004689.1 | + | 27589 | 0.67 | 0.592118 |
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Target: 5'- -aCGcCACUGCGAGUAUcGCCUuCGCGa -3' miRNA: 3'- aaGC-GUGACGUUCGUGaCGGGuGUGU- -5' |
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| 20908 | 3' | -55.9 | NC_004689.1 | + | 53529 | 0.67 | 0.581114 |
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Target: 5'- cUCGCGCUGCGuGGCACUugaaacaccGCUCGCuGCu -3' miRNA: 3'- aAGCGUGACGU-UCGUGA---------CGGGUG-UGu -5' |
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| 20908 | 3' | -55.9 | NC_004689.1 | + | 36394 | 0.73 | 0.282319 |
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Target: 5'- aUCGCGCuUGCAGGCGCaGggaaucgcguCCCACGCAc -3' miRNA: 3'- aAGCGUG-ACGUUCGUGaC----------GGGUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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