Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20908 | 5' | -57.7 | NC_004689.1 | + | 64628 | 0.66 | 0.668627 |
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Target: 5'- aCCUCgCGGUGuGguGUa-GCugUCGCGCAa -3' miRNA: 3'- -GGAG-GCCACuCguUAggCG--AGCGCGU- -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 63010 | 0.66 | 0.635808 |
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Target: 5'- uCCaUCCGGUGAGUGaccgaacGUCCaccgaggaccaaGC-CGCGCAc -3' miRNA: 3'- -GG-AGGCCACUCGU-------UAGG------------CGaGCGCGU- -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 64877 | 0.67 | 0.626262 |
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Target: 5'- cCCUCugCGGUGAcCuGUCC-CUUGCGCAc -3' miRNA: 3'- -GGAG--GCCACUcGuUAGGcGAGCGCGU- -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 48177 | 0.67 | 0.594506 |
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Target: 5'- cCCguaCgCGGUcuGAGUAGUCCGCUgGCGgGu -3' miRNA: 3'- -GGa--G-GCCA--CUCGUUAGGCGAgCGCgU- -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 28646 | 0.67 | 0.594506 |
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Target: 5'- gCCggucuggCCGGUGAGuCGAgcgaagCCGUUCcacaGCGCAu -3' miRNA: 3'- -GGa------GGCCACUC-GUUa-----GGCGAG----CGCGU- -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 58945 | 0.68 | 0.531986 |
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Target: 5'- cUCUCCGGUGGGgAugucuggggucAUCUGCUCaGCGa- -3' miRNA: 3'- -GGAGGCCACUCgU-----------UAGGCGAG-CGCgu -5' |
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| 20908 | 5' | -57.7 | NC_004689.1 | + | 13329 | 0.69 | 0.491635 |
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Target: 5'- aCUCCGGUG-GCucaccggcCUGUUCGUGCGg -3' miRNA: 3'- gGAGGCCACuCGuua-----GGCGAGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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