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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2091 | 5' | -48.7 | NC_001348.1 | + | 21923 | 0.66 | 0.996594 |
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Target: 5'- --cGGUUaGUAAaccUCCCAACCCAg--- -3' miRNA: 3'- auaCCAAgCAUU---AGGGUUGGGUguac -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 103693 | 0.66 | 0.996594 |
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Target: 5'- cAUGGUUCGUgGGUCUUGAuuaauuCCCACAg- -3' miRNA: 3'- aUACCAAGCA-UUAGGGUU------GGGUGUac -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 35948 | 0.67 | 0.994449 |
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Target: 5'- --cGGUU-GU-AUCCUgAACCCGCAUGg -3' miRNA: 3'- auaCCAAgCAuUAGGG-UUGGGUGUAC- -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 8511 | 0.68 | 0.986784 |
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Target: 5'- -------aGUAAUCCCAacuaaacGCCCACGUGa -3' miRNA: 3'- auaccaagCAUUAGGGU-------UGGGUGUAC- -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 44981 | 0.68 | 0.981058 |
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Target: 5'- cAUGG--CGUAGUCCgCAACCCAUuccgGUGu -3' miRNA: 3'- aUACCaaGCAUUAGG-GUUGGGUG----UAC- -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 2512 | 0.69 | 0.978705 |
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Target: 5'- -uUGGUUugaaagcaaUGUAAUCCCG-CCCAUAUa -3' miRNA: 3'- auACCAA---------GCAUUAGGGUuGGGUGUAc -5' |
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| 2091 | 5' | -48.7 | NC_001348.1 | + | 92487 | 1.09 | 0.011454 |
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Target: 5'- uUAUGGUUCGUAAUCCCAACCCACAUGg -3' miRNA: 3'- -AUACCAAGCAUUAGGGUUGGGUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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