Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20912 | 3' | -48.8 | NC_004689.1 | + | 53253 | 0.66 | 0.98762 |
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Target: 5'- cGCCGuAGUCGuAGUuguccUCGuCGUCGuCGGu -3' miRNA: 3'- aCGGCuUCAGC-UUA-----AGCuGUAGU-GCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 28638 | 0.67 | 0.981861 |
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Target: 5'- gGCCGGugAGUCGAgcgaagccGUUCcACAgcgcauUCACGGu -3' miRNA: 3'- aCGGCU--UCAGCU--------UAAGcUGU------AGUGCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 8000 | 0.68 | 0.971318 |
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Target: 5'- gGCCGGugAGUUGAA-UCG-CuUCACGGc -3' miRNA: 3'- aCGGCU--UCAGCUUaAGCuGuAGUGCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 13886 | 0.68 | 0.971318 |
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Target: 5'- aGCCGGAGaCGggUcgcccUCGGCGaaaUCGuCGGu -3' miRNA: 3'- aCGGCUUCaGCuuA-----AGCUGU---AGU-GCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 50081 | 0.68 | 0.968108 |
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Target: 5'- gUGgCG-GGUCGAAcgaGugGUCACGGa -3' miRNA: 3'- -ACgGCuUCAGCUUaagCugUAGUGCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 41944 | 0.68 | 0.956962 |
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Target: 5'- gGCCGGugccuucguGGUCGGAgcacCGACcggCACGGc -3' miRNA: 3'- aCGGCU---------UCAGCUUaa--GCUGua-GUGCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 17375 | 0.7 | 0.921438 |
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Target: 5'- cGCCGAugauguAGUCGAAg--GGCAgcuugaacUCGCGGg -3' miRNA: 3'- aCGGCU------UCAGCUUaagCUGU--------AGUGCC- -5' |
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| 20912 | 3' | -48.8 | NC_004689.1 | + | 64238 | 0.75 | 0.696268 |
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Target: 5'- uUGCCGugauGUCGAAUUCGACuu--CGGc -3' miRNA: 3'- -ACGGCuu--CAGCUUAAGCUGuaguGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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