miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20912 5' -54.8 NC_004689.1 + 14365 0.66 0.769604
Target:  5'- uUCCCUugcGAUGCaa--CGCAAGUUCAg -3'
miRNA:   3'- uAGGGA---CUGCGgaugGCGUUCAAGUg -5'
20912 5' -54.8 NC_004689.1 + 31502 0.68 0.659784
Target:  5'- gAUCCCccacacgucugccaUGuCGCCaugGCCGCGAuuGUUCGCa -3'
miRNA:   3'- -UAGGG--------------ACuGCGGa--UGGCGUU--CAAGUG- -5'
20912 5' -54.8 NC_004689.1 + 30335 0.69 0.608024
Target:  5'- gGUUCCUGuuccuuCGgCUGCCGCGAucauGUUCGCc -3'
miRNA:   3'- -UAGGGACu-----GCgGAUGGCGUU----CAAGUG- -5'
20912 5' -54.8 NC_004689.1 + 8275 0.69 0.58608
Target:  5'- cGUUCCUGACGCUugcgcucacggUACCGcCGAGacCGCa -3'
miRNA:   3'- -UAGGGACUGCGG-----------AUGGC-GUUCaaGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.