Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20915 | 5' | -53.6 | NC_004689.1 | + | 47242 | 0.66 | 0.874332 |
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Target: 5'- cGGgg-GUCUGaCuCGGCGGGGGCAGUc -3' miRNA: 3'- -CCaagCAGACgGcGUUGCUCCUGUCG- -5' |
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| 20915 | 5' | -53.6 | NC_004689.1 | + | 42906 | 0.66 | 0.857663 |
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Target: 5'- gGGUUCGUa-GUCcaucaguGCAGCGAaugccuccaccaGGACGGCg -3' miRNA: 3'- -CCAAGCAgaCGG-------CGUUGCU------------CCUGUCG- -5' |
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| 20915 | 5' | -53.6 | NC_004689.1 | + | 10607 | 0.67 | 0.84171 |
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Target: 5'- cGGagCGcCUGCCGgaGACGuugcGGGCGGUg -3' miRNA: 3'- -CCaaGCaGACGGCg-UUGCu---CCUGUCG- -5' |
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| 20915 | 5' | -53.6 | NC_004689.1 | + | 54323 | 0.67 | 0.824084 |
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Target: 5'- cGGUagUCuUCUGCCGCAGC-AGauuCGGCg -3' miRNA: 3'- -CCA--AGcAGACGGCGUUGcUCcu-GUCG- -5' |
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| 20915 | 5' | -53.6 | NC_004689.1 | + | 27387 | 0.69 | 0.725809 |
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Target: 5'- ----aGUCUGuaCCGCuucuGCGAuGGGCAGCa -3' miRNA: 3'- ccaagCAGAC--GGCGu---UGCU-CCUGUCG- -5' |
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| 20915 | 5' | -53.6 | NC_004689.1 | + | 4172 | 0.69 | 0.692993 |
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Target: 5'- --aUCGUCUugggGCaaCGCGugcugaucaucauGCGAGGACGGCg -3' miRNA: 3'- ccaAGCAGA----CG--GCGU-------------UGCUCCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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