miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20916 3' -55.4 NC_004689.1 + 50533 0.66 0.8041
Target:  5'- -gGCCUGuccGCGuucGACCggcUCACGCCAGAa -3'
miRNA:   3'- ggUGGGCua-CGU---CUGG---AGUGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 70370 0.66 0.78508
Target:  5'- gCCGUCCGGUGUcuuGGCCgu-CACCAGGc -3'
miRNA:   3'- -GGUGGGCUACGu--CUGGaguGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 1964 0.66 0.765452
Target:  5'- aCCAgUCGGguUGUAcuGGCCUC-CACCGGAc -3'
miRNA:   3'- -GGUgGGCU--ACGU--CUGGAGuGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 38333 0.67 0.7453
Target:  5'- -uGCCCGAUucaCGGACCUCG-ACCGGc -3'
miRNA:   3'- ggUGGGCUAc--GUCUGGAGUgUGGUCu -5'
20916 3' -55.4 NC_004689.1 + 33452 0.67 0.72367
Target:  5'- gUACCCGccAUGCGGguccauguucGCCaggaacaUCACGCCGGAa -3'
miRNA:   3'- gGUGGGC--UACGUC----------UGG-------AGUGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 30473 0.67 0.703766
Target:  5'- aCCACcaCCGA-GCAgaauguaaucGACCagCACACCAGGg -3'
miRNA:   3'- -GGUG--GGCUaCGU----------CUGGa-GUGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 23930 0.68 0.693189
Target:  5'- gCCACCUGAccgGCAG-CCUgaAUugCAGAg -3'
miRNA:   3'- -GGUGGGCUa--CGUCuGGAg-UGugGUCU- -5'
20916 3' -55.4 NC_004689.1 + 42896 0.68 0.66116
Target:  5'- uCCAUCa-GUGCAGcgaauGCCUC-CACCAGGa -3'
miRNA:   3'- -GGUGGgcUACGUC-----UGGAGuGUGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 46632 0.68 0.639661
Target:  5'- uCCACUCGAUcuuguuggacgGCAGuuccgGCUUCACGuCCAGAc -3'
miRNA:   3'- -GGUGGGCUA-----------CGUC-----UGGAGUGU-GGUCU- -5'
20916 3' -55.4 NC_004689.1 + 42706 0.69 0.628898
Target:  5'- cCCAUUCGAUgucGCGGACCaUUGC-CCAGAc -3'
miRNA:   3'- -GGUGGGCUA---CGUCUGG-AGUGuGGUCU- -5'
20916 3' -55.4 NC_004689.1 + 52406 0.71 0.512503
Target:  5'- aCAgCCGGUGCAGcuGCCUUuuuCGCCGGu -3'
miRNA:   3'- gGUgGGCUACGUC--UGGAGu--GUGGUCu -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.