Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2092 | 5' | -52.8 | NC_001348.1 | + | 62930 | 0.68 | 0.894752 |
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Target: 5'- cGUUAAGGCGCcuUGAUcauaCCGCCCCccacgaGCAUGa -3' miRNA: 3'- -CAGUUUUGCG--ACUG----GGUGGGG------UGUAC- -5' |
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| 2092 | 5' | -52.8 | NC_001348.1 | + | 113430 | 0.72 | 0.68392 |
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Target: 5'- -cCuuGACGUUGACCCGCUuuaaugaCCACAUGu -3' miRNA: 3'- caGuuUUGCGACUGGGUGG-------GGUGUAC- -5' |
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| 2092 | 5' | -52.8 | NC_001348.1 | + | 93089 | 0.74 | 0.578604 |
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Target: 5'- gGUCA--GCGUuuUGACCUGCCCCACGg- -3' miRNA: 3'- -CAGUuuUGCG--ACUGGGUGGGGUGUac -5' |
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| 2092 | 5' | -52.8 | NC_001348.1 | + | 105715 | 0.75 | 0.547124 |
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Target: 5'- gGUCGAAGCGCgGcACCCagGCCCCAUcgGu -3' miRNA: 3'- -CAGUUUUGCGaC-UGGG--UGGGGUGuaC- -5' |
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| 2092 | 5' | -52.8 | NC_001348.1 | + | 124077 | 0.75 | 0.547124 |
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Target: 5'- gGUCGAAGCGCgGcACCCagGCCCCAUcgGu -3' miRNA: 3'- -CAGUUUUGCGaC-UGGG--UGGGGUGuaC- -5' |
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| 2092 | 5' | -52.8 | NC_001348.1 | + | 92950 | 1.09 | 0.004387 |
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Target: 5'- gGUCAAAACGCUGACCCACCCCACAUGa -3' miRNA: 3'- -CAGUUUUGCGACUGGGUGGGGUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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