Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20921 | 5' | -45.7 | NC_004690.1 | + | 23570 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 23660 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 26179 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 95157 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 95247 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 26045 | 0.66 | 0.999752 |
Target: 5'- -aCUUgACUCUGAGUuucguUUGGUUgAUGUc -3' miRNA: 3'- uaGAGaUGAGACUCA-----AACCAAgUAUA- -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 84735 | 0.71 | 0.980129 |
Target: 5'- -aCUUgACUCUGAGUUUGGUUgGa-- -3' miRNA: 3'- uaGAGaUGAGACUCAAACCAAgUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 23548 | 0.71 | 0.980129 |
Target: 5'- -aCUUgACUCUGAGUUUGGUUgGa-- -3' miRNA: 3'- uaGAGaUGAGACUCAAACCAAgUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 23443 | 0.73 | 0.952682 |
Target: 5'- aAUCUCgACUCUGAGUUUcGUUCGa-- -3' miRNA: 3'- -UAGAGaUGAGACUCAAAcCAAGUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 84840 | 0.73 | 0.952682 |
Target: 5'- aAUCUCgACUCUGAGUUUcGUUCGg-- -3' miRNA: 3'- -UAGAGaUGAGACUCAAAcCAAGUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 25940 | 0.76 | 0.868844 |
Target: 5'- aAUCUCUAC-CUGAGUUUGG-UCGg-- -3' miRNA: 3'- -UAGAGAUGaGACUCAAACCaAGUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 26285 | 0.8 | 0.685477 |
Target: 5'- aAUCUCgACUCUGAGUUUGGUUgGa-- -3' miRNA: 3'- -UAGAGaUGAGACUCAAACCAAgUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 25796 | 0.85 | 0.420692 |
Target: 5'- aAUCUUgACUCUGAGUUUGGUUCAa-- -3' miRNA: 3'- -UAGAGaUGAGACUCAAACCAAGUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 23765 | 0.87 | 0.346258 |
Target: 5'- aAUCUCUACUCUGAGUUUGG-UCGg-- -3' miRNA: 3'- -UAGAGAUGAGACUCAAACCaAGUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 95352 | 0.89 | 0.246487 |
Target: 5'- aAUCUCUACUCUGAGUUUGGUUgGg-- -3' miRNA: 3'- -UAGAGAUGAGACUCAAACCAAgUaua -5' |
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20921 | 5' | -45.7 | NC_004690.1 | + | 23675 | 1.04 | 0.03526 |
Target: 5'- aAUCUCUACUCUGAGUUUGGUUCAUAUc -3' miRNA: 3'- -UAGAGAUGAGACUCAAACCAAGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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