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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
20924 | 5' | -51.8 | NC_004690.1 | + | 80727 | 0.67 | 0.935429 |
Target: 5'- --uUGAUUACACucucaaaagaCCGaCGACAGAUAGg -3' miRNA: 3'- aauACUGAUGUG----------GGC-GCUGUCUGUCg -5' |
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20924 | 5' | -51.8 | NC_004690.1 | + | 71315 | 0.69 | 0.892398 |
Target: 5'- ---aGACguucgACAgCCCGU-ACAGACGGCg -3' miRNA: 3'- aauaCUGa----UGU-GGGCGcUGUCUGUCG- -5' |
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20924 | 5' | -51.8 | NC_004690.1 | + | 39638 | 0.78 | 0.418367 |
Target: 5'- -gAUGugUACGCCCGCGACuauAUAGUa -3' miRNA: 3'- aaUACugAUGUGGGCGCUGuc-UGUCG- -5' |
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20924 | 5' | -51.8 | NC_004690.1 | + | 56800 | 1.09 | 0.004706 |
Target: 5'- uUUAUGACUACACCCGCGACAGACAGCc -3' miRNA: 3'- -AAUACUGAUGUGGGCGCUGUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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