Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20925 | 3' | -45 | NC_004690.1 | + | 53945 | 0.66 | 0.999942 |
Target: 5'- aUAUGUCaAACCAAAUUUAcGAuGUCAAGu -3' miRNA: 3'- -GUAUAGgUUGGUUUGAGU-CU-CAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 15588 | 0.67 | 0.999896 |
Target: 5'- aAUGUCaccuaAACgAGACUUGGGGUCGAu -3' miRNA: 3'- gUAUAGg----UUGgUUUGAGUCUCAGUUc -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 44340 | 0.71 | 0.994818 |
Target: 5'- uGUcgUCGGCCAGACaaAGAGUCGAa -3' miRNA: 3'- gUAuaGGUUGGUUUGagUCUCAGUUc -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23713 | 0.71 | 0.993919 |
Target: 5'- cCAUAUUgAACCAAAUUUAGAG-CAAu -3' miRNA: 3'- -GUAUAGgUUGGUUUGAGUCUCaGUUc -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23536 | 0.71 | 0.992898 |
Target: 5'- gAUA-UgAACCAAACUCAGAGUaGAGa -3' miRNA: 3'- gUAUaGgUUGGUUUGAGUCUCAgUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 25754 | 0.77 | 0.898372 |
Target: 5'- aGUAUgacaUCAACCAAACgaaagUCAGAGUCAAGu -3' miRNA: 3'- gUAUA----GGUUGGUUUG-----AGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23709 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23799 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 26319 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 95296 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 95386 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 25906 | 0.79 | 0.803683 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCAAGu -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23586 | 0.83 | 0.62968 |
Target: 5'- aCAUAgcUCgAACgAAACUCAGAGUCGAGa -3' miRNA: 3'- -GUAU--AGgUUGgUUUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 84696 | 0.88 | 0.38635 |
Target: 5'- --gAUCCAACCcgaacgAAACUCAGAGUCGAGa -3' miRNA: 3'- guaUAGGUUGG------UUUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 26082 | 0.89 | 0.333524 |
Target: 5'- cCAUAUcCCGACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23623 | 0.89 | 0.333524 |
Target: 5'- cCAUAUcCCGACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 25934 | 0.89 | 0.325252 |
Target: 5'- --gAUUgAACCAAACUCAGAGUCAAGa -3' miRNA: 3'- guaUAGgUUGGUUUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 95210 | 0.9 | 0.309167 |
Target: 5'- cCAUAUcCCAACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 84874 | 1.1 | 0.022029 |
Target: 5'- cCAUAUCCAACCAAACUCAGAGUCAAGu -3' miRNA: 3'- -GUAUAGGUUGGUUUGAGUCUCAGUUC- -5' |
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20925 | 3' | -45 | NC_004690.1 | + | 23409 | 1.1 | 0.022029 |
Target: 5'- cCAUAUCCAACCAAACUCAGAGUCAAGu -3' miRNA: 3'- -GUAUAGGUUGGUUUGAGUCUCAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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