Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20929 | 3' | -44.4 | NC_004690.1 | + | 15585 | 0.68 | 0.999832 |
Target: 5'- gUCAcCUAAACGAGAcUUGGGGUCGAu -3' miRNA: 3'- -AGUuGGUUUGCUUUcAGUCUCAGUUc -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 27167 | 0.69 | 0.99942 |
Target: 5'- uUCAAUCuGAUGGcaaugAAGUCAGAccGUCGAGa -3' miRNA: 3'- -AGUUGGuUUGCU-----UUCAGUCU--CAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 23495 | 0.7 | 0.998623 |
Target: 5'- gCAAcCCGAACGAAAuUCAGAG-CAAa -3' miRNA: 3'- aGUU-GGUUUGCUUUcAGUCUCaGUUc -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 25930 | 0.7 | 0.997958 |
Target: 5'- -gAACCAAAC-----UCAGAGUCAAGa -3' miRNA: 3'- agUUGGUUUGcuuucAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 23415 | 0.75 | 0.968195 |
Target: 5'- cCAACCAAAC-----UCAGAGUCAAGu -3' miRNA: 3'- aGUUGGUUUGcuuucAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 84868 | 0.75 | 0.968195 |
Target: 5'- cCAACCAAAC-----UCAGAGUCAAGu -3' miRNA: 3'- aGUUGGUUUGcuuucAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 26149 | 0.75 | 0.960913 |
Target: 5'- cCAACCAAAC-----UCAGAGUCGAGa -3' miRNA: 3'- aGUUGGUUUGcuuucAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 23584 | 0.83 | 0.679093 |
Target: 5'- aUAGCuCGAACGAAAcUCAGAGUCGAGa -3' miRNA: 3'- aGUUG-GUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 84699 | 0.86 | 0.522026 |
Target: 5'- cCAAcCCGAACGAAAcUCAGAGUCGAGa -3' miRNA: 3'- aGUU-GGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 25906 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 95386 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 95296 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 26319 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 23799 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 23709 | 0.98 | 0.138076 |
Target: 5'- aUCAACCAAACGAAAcUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUcAGUCUCAGUUC- -5' |
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20929 | 3' | -44.4 | NC_004690.1 | + | 25762 | 1.11 | 0.023101 |
Target: 5'- aUCAACCAAACGAAAGUCAGAGUCAAGu -3' miRNA: 3'- -AGUUGGUUUGCUUUCAGUCUCAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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