Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20929 | 5' | -45 | NC_004690.1 | + | 23765 | 0.72 | 0.98516 |
Target: 5'- aAUCUcuACUCUGAGUUUGG-UCGGg- -3' miRNA: 3'- -UAGAacUGAGACUCAAACCaAGUUag -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 25901 | 0.74 | 0.949514 |
Target: 5'- -aCUUGACUCUGAcuuucGUUUGGUUgauGUCa -3' miRNA: 3'- uaGAACUGAGACU-----CAAACCAAgu-UAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 95352 | 0.75 | 0.939506 |
Target: 5'- aAUCUcuACUCUGAGUUUGGUUgGGa- -3' miRNA: 3'- -UAGAacUGAGACUCAAACCAAgUUag -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 23443 | 0.77 | 0.871601 |
Target: 5'- aAUCUcGACUCUGAGUUUcGUUCGAg- -3' miRNA: 3'- -UAGAaCUGAGACUCAAAcCAAGUUag -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 23570 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 23660 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 26179 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 95157 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 95247 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 26045 | 0.78 | 0.817647 |
Target: 5'- -aCUUGACUCUGAGUUUcGUUUggUUg -3' miRNA: 3'- uaGAACUGAGACUCAAAcCAAGuuAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 84840 | 0.78 | 0.80785 |
Target: 5'- aAUCUcGACUCUGAGUUUcguucgGGUUgGAUCa -3' miRNA: 3'- -UAGAaCUGAGACUCAAA------CCAAgUUAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 23675 | 0.85 | 0.46545 |
Target: 5'- aAUCUcuACUCUGAGUUUGGUUCAuAUCa -3' miRNA: 3'- -UAGAacUGAGACUCAAACCAAGU-UAG- -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 26285 | 0.86 | 0.424642 |
Target: 5'- aAUCUcGACUCUGAGUUUGGUUgGAUa -3' miRNA: 3'- -UAGAaCUGAGACUCAAACCAAgUUAg -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 84735 | 0.9 | 0.263129 |
Target: 5'- -aCUUGACUCUGAGUUUGGUUgGAUa -3' miRNA: 3'- uaGAACUGAGACUCAAACCAAgUUAg -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 23548 | 0.9 | 0.263129 |
Target: 5'- -aCUUGACUCUGAGUUUGGUUgGAUa -3' miRNA: 3'- uaGAACUGAGACUCAAACCAAgUUAg -5' |
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20929 | 5' | -45 | NC_004690.1 | + | 25796 | 1.1 | 0.018763 |
Target: 5'- aAUCUUGACUCUGAGUUUGGUUCAAUCa -3' miRNA: 3'- -UAGAACUGAGACUCAAACCAAGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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