Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20930 | 3' | -46.8 | NC_004690.1 | + | 25754 | 0.66 | 0.999631 |
Target: 5'- aGUAUgacaUCAACCAAACgaaagUCAGAGUCa-- -3' miRNA: 3'- gUAUA----GGUUGGUUUG-----AGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 26319 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 95296 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 95386 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23799 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 25906 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23709 | 0.68 | 0.996532 |
Target: 5'- ----aUCAACCAaacgaAACUCAGAGUCa-- -3' miRNA: 3'- guauaGGUUGGU-----UUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 15588 | 0.69 | 0.99516 |
Target: 5'- aAUGUCaccuaAACgAGACUUGGGGUCGAu -3' miRNA: 3'- gUAUAGg----UUGgUUUGAGUCUCAGCUc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23536 | 0.72 | 0.962454 |
Target: 5'- gAUA-UgAACCAAACUCAGAGUaGAGa -3' miRNA: 3'- gUAUaGgUUGGUUUGAGUCUCAgCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 44338 | 0.72 | 0.954457 |
Target: 5'- uGUGUCguCGGCCAGACaaAGAGUCGAa -3' miRNA: 3'- gUAUAG--GUUGGUUUGagUCUCAGCUc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 25934 | 0.77 | 0.795179 |
Target: 5'- --gAUUgAACCAAACUCAGAGUCaAGa -3' miRNA: 3'- guaUAGgUUGGUUUGAGUCUCAGcUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23586 | 0.84 | 0.451769 |
Target: 5'- aCAUAgcUCgAACgAAACUCAGAGUCGAGa -3' miRNA: 3'- -GUAU--AGgUUGgUUUGAGUCUCAGCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 84696 | 0.89 | 0.25867 |
Target: 5'- --gAUCCAACCcgaacgAAACUCAGAGUCGAGa -3' miRNA: 3'- guaUAGGUUGG------UUUGAGUCUCAGCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23623 | 0.89 | 0.245502 |
Target: 5'- cCAUAUcCCGACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 26082 | 0.89 | 0.245502 |
Target: 5'- cCAUAUcCCGACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 95210 | 0.9 | 0.226798 |
Target: 5'- cCAUAUcCCAACCAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUAUA-GGUUGGUUUGAGUCUCAgCUC- -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 84874 | 0.98 | 0.080375 |
Target: 5'- cCAUAUCCAACCAAACUCAGAGUCa-- -3' miRNA: 3'- -GUAUAGGUUGGUUUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 23409 | 0.98 | 0.080375 |
Target: 5'- cCAUAUCCAACCAAACUCAGAGUCa-- -3' miRNA: 3'- -GUAUAGGUUGGUUUGAGUCUCAGcuc -5' |
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20930 | 3' | -46.8 | NC_004690.1 | + | 26143 | 1.11 | 0.0135 |
Target: 5'- cCAUAUCCAACCAAACUCAGAGUCGAGa -3' miRNA: 3'- -GUAUAGGUUGGUUUGAGUCUCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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