Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20931 | 3' | -49.4 | NC_004690.1 | + | 27168 | 0.67 | 0.991738 |
Target: 5'- uCAAUCUGAugGcaauGAAgUCAGAccGUCGAGa -3' miRNA: 3'- -GUUGGGCUugC----UUUgAGUCU--CAGCUC- -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 23623 | 0.67 | 0.989128 |
Target: 5'- cCAuauCCCG-ACcAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUu--GGGCuUGcUUUGAGUCUCAgCUC- -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 26082 | 0.67 | 0.989128 |
Target: 5'- cCAuauCCCG-ACcAAACUCAGAGUaGAGa -3' miRNA: 3'- -GUu--GGGCuUGcUUUGAGUCUCAgCUC- -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 25763 | 0.7 | 0.940509 |
Target: 5'- uCAACCa-AACGAAAgUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUgAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 26146 | 0.74 | 0.828155 |
Target: 5'- -uAUCC-AACcAAACUCAGAGUCGAGa -3' miRNA: 3'- guUGGGcUUGcUUUGAGUCUCAGCUC- -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 15583 | 0.75 | 0.771397 |
Target: 5'- -cACCUaAACGAGACUUGGGGUCGAu -3' miRNA: 3'- guUGGGcUUGCUUUGAGUCUCAGCUc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 25907 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 95385 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 95295 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 26318 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 23798 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 23708 | 0.78 | 0.5911 |
Target: 5'- uCAACCa-AACGAAACUCAGAGUCa-- -3' miRNA: 3'- -GUUGGgcUUGCUUUGAGUCUCAGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 23495 | 0.83 | 0.364981 |
Target: 5'- gCAACCCGAACGAAAUUCAGAG-Ca-- -3' miRNA: 3'- -GUUGGGCUUGCUUUGAGUCUCaGcuc -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 23584 | 1.02 | 0.028888 |
Target: 5'- aUAGCUCGAACGAAACUCAGAGUCGAGa -3' miRNA: 3'- -GUUGGGCUUGCUUUGAGUCUCAGCUC- -5' |
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20931 | 3' | -49.4 | NC_004690.1 | + | 84699 | 1.1 | 0.00872 |
Target: 5'- cCAACCCGAACGAAACUCAGAGUCGAGa -3' miRNA: 3'- -GUUGGGCUUGCUUUGAGUCUCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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