Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20948 | 5' | -47.1 | NC_004718.3 | + | 8903 | 0.66 | 0.948022 |
Target: 5'- aGUGAUuuUGCUACCucuGCUugcguucuuGCUGCUGAg -3' miRNA: 3'- -CGUUAuuAUGGUGGu--CGG---------UGAUGACU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 23988 | 0.66 | 0.924743 |
Target: 5'- aCAGUGuUGCCACCucuGCuCACUGaUGAu -3' miRNA: 3'- cGUUAUuAUGGUGGu--CG-GUGAUgACU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 24046 | 0.67 | 0.911039 |
Target: 5'- uUAGUGGUACU----GCCACUGCUGGa -3' miRNA: 3'- cGUUAUUAUGGugguCGGUGAUGACU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 10591 | 0.69 | 0.843045 |
Target: 5'- -----uGUACCugCAGCCugUGCa-- -3' miRNA: 3'- cguuauUAUGGugGUCGGugAUGacu -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 1255 | 0.7 | 0.779134 |
Target: 5'- gGCAGacGUGCgACUuucugaaAGCCACUugUGAa -3' miRNA: 3'- -CGUUauUAUGgUGG-------UCGGUGAugACU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 12645 | 0.76 | 0.415833 |
Target: 5'- uGCGGcUGGUACCACacaaaCAGCUugUACUGAu -3' miRNA: 3'- -CGUU-AUUAUGGUG-----GUCGGugAUGACU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 4149 | 0.77 | 0.375177 |
Target: 5'- uCAGUAGUGCCACCAGCCuuuUUGgaGGg -3' miRNA: 3'- cGUUAUUAUGGUGGUCGGu--GAUgaCU- -5' |
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20948 | 5' | -47.1 | NC_004718.3 | + | 9377 | 1.13 | 0.00171 |
Target: 5'- gGCAAUAAUACCACCAGCCACUACUGAa -3' miRNA: 3'- -CGUUAUUAUGGUGGUCGGUGAUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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