Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20952 | 3' | -47.8 | NC_004718.3 | + | 28594 | 0.66 | 0.898626 |
Target: 5'- uGCCaaaagGCuucuaCGCAGAGGgAAGcAGAGGCg -3' miRNA: 3'- -CGGaaa--CG-----GCGUCUCUgUUU-UCUUCG- -5' |
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20952 | 3' | -47.8 | NC_004718.3 | + | 13462 | 0.66 | 0.920234 |
Target: 5'- uGCCUgUGCCGCAcGGugu-AAGAcgGGCu -3' miRNA: 3'- -CGGAaACGGCGUcUCuguuUUCU--UCG- -5' |
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20952 | 3' | -47.8 | NC_004718.3 | + | 28511 | 0.68 | 0.846723 |
Target: 5'- uGCCUUcuuuguuagcGCCGUAGGGAagu--GAAGCu -3' miRNA: 3'- -CGGAAa---------CGGCGUCUCUguuuuCUUCG- -5' |
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20952 | 3' | -47.8 | NC_004718.3 | + | 4913 | 0.69 | 0.806458 |
Target: 5'- aCCUc--CCGCAGGGAUAAGAGAc-- -3' miRNA: 3'- cGGAaacGGCGUCUCUGUUUUCUucg -5' |
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20952 | 3' | -47.8 | NC_004718.3 | + | 29232 | 1.17 | 0.000766 |
Target: 5'- aGCCUUUGCCGCAGAGACAAAAGAAGCa -3' miRNA: 3'- -CGGAAACGGCGUCUCUGUUUUCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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