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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20962 | 3' | -60.8 | NC_004724.1 | + | 11082 | 0.67 | 0.140448 |
Target: 5'- aACCUUCCgcgGCUGCUUUaucagCGUGCAAUCUg -3' miRNA: 3'- -UGGGAGGa--UGGCGGAG-----GCGCGUUGGG- -5' |
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20962 | 3' | -60.8 | NC_004724.1 | + | 13881 | 0.76 | 0.025669 |
Target: 5'- gACCCUCCUACCGCgauucCCGUGCcggAACUCa -3' miRNA: 3'- -UGGGAGGAUGGCGga---GGCGCG---UUGGG- -5' |
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20962 | 3' | -60.8 | NC_004724.1 | + | 5834 | 1.11 | 0.000035 |
Target: 5'- cACCCUCCUACCGCCUCCGCGCAACCCa -3' miRNA: 3'- -UGGGAGGAUGGCGGAGGCGCGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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