miRNA: 3'- uaaCGCGGCUUUUUGc-GAA---CuAGCUG- -5'
miRNA: 3'- uAACGCGGCUUuu-UGCGaa--CUAGCUG- -5'
miRNA: 3'- uaaCGC-GGCUUUUu-----GCGAACUAGCuG- -5'
miRNA: 3'- -UAACGCGGCUUUUUGCGAACUAGCUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20969 | 5' | -48.2 | NC_004724.1 | + | 926 | 0.66 | 0.72316 |
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Target: 5'- --cGCGCCGAGAAGCuuCUUucaGcUCGGCg -3' miRNA: 3'- uaaCGCGGCUUUUUGc-GAA---CuAGCUG- -5' |
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| 20969 | 5' | -48.2 | NC_004724.1 | + | 3860 | 0.67 | 0.710715 |
|
Target: 5'- cUUGCGUCGGGuguGCGCagcaGGUUGGCu -3' miRNA: 3'- uAACGCGGCUUuu-UGCGaa--CUAGCUG- -5' |
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| 20969 | 5' | -48.2 | NC_004724.1 | + | 6740 | 0.67 | 0.710715 |
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Target: 5'- --gGCGuUCGGAGAuuccuuCGCUUGAUCG-Cg -3' miRNA: 3'- uaaCGC-GGCUUUUu-----GCGAACUAGCuG- -5' |
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| 20969 | 5' | -48.2 | NC_004724.1 | + | 15861 | 1.1 | 0.000884 |
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Target: 5'- gAUUGCGCCGAAAAACGCUUGAUCGACa -3' miRNA: 3'- -UAACGCGGCUUUUUGCGAACUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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