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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20970 | 5' | -51.6 | NC_004725.1 | + | 3385 | 0.69 | 0.106518 |
Target: 5'- -cGUUGGCGGCGUUuacaCCUACUacaGCUGGCa -3' miRNA: 3'- caCAAUUGUCGUAA----GGGUGG---CGGUCG- -5' |
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20970 | 5' | -51.6 | NC_004725.1 | + | 485 | 1.11 | 0.000033 |
Target: 5'- gGUGUUAACAGCAUUCCCACCGCCAGCc -3' miRNA: 3'- -CACAAUUGUCGUAAGGGUGGCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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