Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20982 | 3' | -54.3 | NC_004735.1 | + | 10532 | 0.66 | 0.883848 |
Target: 5'- uGUCGUcaagaAUGUAGGGAGUAGCCGg -3' miRNA: 3'- -UAGCGuacggUACGUCUUUCGUCGGC- -5' |
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20982 | 3' | -54.3 | NC_004735.1 | + | 101360 | 0.67 | 0.868797 |
Target: 5'- -gCGCuauUGCCGUGguGGcGGGCGGUCu -3' miRNA: 3'- uaGCGu--ACGGUACguCU-UUCGUCGGc -5' |
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20982 | 3' | -54.3 | NC_004735.1 | + | 105151 | 0.67 | 0.84449 |
Target: 5'- uGUCGCuugagcgccuUGCCGUGCAGugGGUAGaCGu -3' miRNA: 3'- -UAGCGu---------ACGGUACGUCuuUCGUCgGC- -5' |
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20982 | 3' | -54.3 | NC_004735.1 | + | 50003 | 0.72 | 0.560979 |
Target: 5'- aAUUGCAccuUGCCAUGCAGGAAGaauuGCUa -3' miRNA: 3'- -UAGCGU---ACGGUACGUCUUUCgu--CGGc -5' |
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20982 | 3' | -54.3 | NC_004735.1 | + | 3429 | 1.07 | 0.003643 |
Target: 5'- gAUCGCAUGCCAUGCAGAAAGCAGCCGu -3' miRNA: 3'- -UAGCGUACGGUACGUCUUUCGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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