miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20982 5' -57.3 NC_004735.1 + 45339 0.66 0.843841
Target:  5'- gGUGUGGuUGAUCGCcugCUggaaGCGccGCUCCCu -3'
miRNA:   3'- gCGUACC-ACUAGCGa--GG----UGC--CGAGGG- -5'
20982 5' -57.3 NC_004735.1 + 72713 0.68 0.759062
Target:  5'- uGCccaaaGUGGUGcuUCGCUuugaugcgaacacccUCACGGCUUCCu -3'
miRNA:   3'- gCG-----UACCACu-AGCGA---------------GGUGCCGAGGG- -5'
20982 5' -57.3 NC_004735.1 + 44799 0.7 0.635563
Target:  5'- uCGCAUGGUGGUgucaagUGCUucUCGCuGCUCCUu -3'
miRNA:   3'- -GCGUACCACUA------GCGA--GGUGcCGAGGG- -5'
20982 5' -57.3 NC_004735.1 + 77427 0.7 0.62537
Target:  5'- gGCGUGGg---UGCUCCACaaacugGGCUaCCCg -3'
miRNA:   3'- gCGUACCacuaGCGAGGUG------CCGA-GGG- -5'
20982 5' -57.3 NC_004735.1 + 68167 0.71 0.564581
Target:  5'- gGCAaauUGGUGAUUGa--CAUGGCUCCa -3'
miRNA:   3'- gCGU---ACCACUAGCgagGUGCCGAGGg -5'
20982 5' -57.3 NC_004735.1 + 3393 1.11 0.001459
Target:  5'- cCGCAUGGUGAUCGCUCCACGGCUCCCc -3'
miRNA:   3'- -GCGUACCACUAGCGAGGUGCCGAGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.