Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20982 | 5' | -57.3 | NC_004735.1 | + | 45339 | 0.66 | 0.843841 |
Target: 5'- gGUGUGGuUGAUCGCcugCUggaaGCGccGCUCCCu -3' miRNA: 3'- gCGUACC-ACUAGCGa--GG----UGC--CGAGGG- -5' |
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20982 | 5' | -57.3 | NC_004735.1 | + | 72713 | 0.68 | 0.759062 |
Target: 5'- uGCccaaaGUGGUGcuUCGCUuugaugcgaacacccUCACGGCUUCCu -3' miRNA: 3'- gCG-----UACCACu-AGCGA---------------GGUGCCGAGGG- -5' |
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20982 | 5' | -57.3 | NC_004735.1 | + | 44799 | 0.7 | 0.635563 |
Target: 5'- uCGCAUGGUGGUgucaagUGCUucUCGCuGCUCCUu -3' miRNA: 3'- -GCGUACCACUA------GCGA--GGUGcCGAGGG- -5' |
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20982 | 5' | -57.3 | NC_004735.1 | + | 77427 | 0.7 | 0.62537 |
Target: 5'- gGCGUGGg---UGCUCCACaaacugGGCUaCCCg -3' miRNA: 3'- gCGUACCacuaGCGAGGUG------CCGA-GGG- -5' |
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20982 | 5' | -57.3 | NC_004735.1 | + | 68167 | 0.71 | 0.564581 |
Target: 5'- gGCAaauUGGUGAUUGa--CAUGGCUCCa -3' miRNA: 3'- gCGU---ACCACUAGCgagGUGCCGAGGg -5' |
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20982 | 5' | -57.3 | NC_004735.1 | + | 3393 | 1.11 | 0.001459 |
Target: 5'- cCGCAUGGUGAUCGCUCCACGGCUCCCc -3' miRNA: 3'- -GCGUACCACUAGCGAGGUGCCGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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