Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20983 | 3' | -51.4 | NC_004735.1 | + | 65573 | 0.67 | 0.962424 |
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Target: 5'- cGGGAUAugcaGCaCCCGGUaucagcggcUCCCCACUUa- -3' miRNA: 3'- -CUCUAU----UGcGGGCUA---------AGGGGUGAAcu -5' |
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| 20983 | 3' | -51.4 | NC_004735.1 | + | 12058 | 0.68 | 0.946131 |
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Target: 5'- cGGGAUaAAUGCCCGAaUUCCCC-Cg--- -3' miRNA: 3'- -CUCUA-UUGCGGGCU-AAGGGGuGaacu -5' |
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| 20983 | 3' | -51.4 | NC_004735.1 | + | 111748 | 0.69 | 0.930713 |
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Target: 5'- -uGAUAagggcacGCGCCagCGAUUCaCCCACUUGu -3' miRNA: 3'- cuCUAU-------UGCGG--GCUAAG-GGGUGAACu -5' |
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| 20983 | 3' | -51.4 | NC_004735.1 | + | 78533 | 0.69 | 0.920026 |
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Target: 5'- aGGAUAGCGCUgGAgUUCCCGCUc-- -3' miRNA: 3'- cUCUAUUGCGGgCUaAGGGGUGAacu -5' |
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| 20983 | 3' | -51.4 | NC_004735.1 | + | 13899 | 0.73 | 0.766386 |
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Target: 5'- -----cACGCCCGAcUCCCCGCgucUGAa -3' miRNA: 3'- cucuauUGCGGGCUaAGGGGUGa--ACU- -5' |
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| 20983 | 3' | -51.4 | NC_004735.1 | + | 15758 | 1.09 | 0.006318 |
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Target: 5'- aGAGAUAACGCCCGAUUCCCCACUUGAc -3' miRNA: 3'- -CUCUAUUGCGGGCUAAGGGGUGAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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