Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20985 | 3' | -63.2 | NC_004735.1 | + | 103436 | 0.66 | 0.599133 |
Target: 5'- aCgGCgGCUCCgauCAgCGGAGUCAGcCCCu -3' miRNA: 3'- -GgCG-CGAGG---GUgGCCUCGGUCuGGGc -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 10046 | 0.66 | 0.551006 |
Target: 5'- aUC-CGUUCCCACCGGuacaaccgGGUCAGACUUu -3' miRNA: 3'- -GGcGCGAGGGUGGCC--------UCGGUCUGGGc -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 56924 | 0.66 | 0.548152 |
Target: 5'- aCGCGCcaucauaccguauaUCCCGUCaGGAGCCGGAUCg- -3' miRNA: 3'- gGCGCG--------------AGGGUGG-CCUCGGUCUGGgc -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 117039 | 0.67 | 0.532069 |
Target: 5'- cCCGUGaUCCCGcaaaucCCGGuGCCGGAUUCa -3' miRNA: 3'- -GGCGCgAGGGU------GGCCuCGGUCUGGGc -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 126529 | 0.71 | 0.33115 |
Target: 5'- uCCGCGCUacaACCGGAucGUCGGugCCGu -3' miRNA: 3'- -GGCGCGAgggUGGCCU--CGGUCugGGC- -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 1074 | 0.76 | 0.143592 |
Target: 5'- aCGCGCUUCCACUGGuGCUugacGAUCCGg -3' miRNA: 3'- gGCGCGAGGGUGGCCuCGGu---CUGGGC- -5' |
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20985 | 3' | -63.2 | NC_004735.1 | + | 29129 | 0.98 | 0.004331 |
Target: 5'- gCCGCGCUCCCACCGGAG-CAGACCCGu -3' miRNA: 3'- -GGCGCGAGGGUGGCCUCgGUCUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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