Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20986 | 5' | -50.6 | NC_004735.1 | + | 25469 | 0.66 | 0.988151 |
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Target: 5'- -gUGAAgcUUGCCgAAGAGGu-GAGCGGGa -3' miRNA: 3'- uaGCUU--AGCGG-UUCUCCuuCUUGCCU- -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 52874 | 0.66 | 0.986531 |
|
Target: 5'- uAUCGAAUCGgCu-GAGGAAauGCGGu -3' miRNA: 3'- -UAGCUUAGCgGuuCUCCUUcuUGCCu -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 73788 | 0.67 | 0.980647 |
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Target: 5'- uUCGGugCGCCcgucGGGAAGAAUGGAc -3' miRNA: 3'- uAGCUuaGCGGuuc-UCCUUCUUGCCU- -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 34317 | 0.67 | 0.980647 |
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Target: 5'- -aCGAucucaagCcCCAGGAGGAAGuGGCGGAa -3' miRNA: 3'- uaGCUua-----GcGGUUCUCCUUC-UUGCCU- -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 11442 | 0.68 | 0.959776 |
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Target: 5'- uUUGGAUCGCUGacGGuGGAAGAGCuGAg -3' miRNA: 3'- uAGCUUAGCGGU--UCuCCUUCUUGcCU- -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 11853 | 0.72 | 0.849855 |
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Target: 5'- aAUUGggUucCGCCAccaucguuugcgGGuGGAAGAACGGAu -3' miRNA: 3'- -UAGCuuA--GCGGU------------UCuCCUUCUUGCCU- -5' |
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| 20986 | 5' | -50.6 | NC_004735.1 | + | 34199 | 1.08 | 0.008851 |
|
Target: 5'- gAUCGAAUCGCCAAGAGGAAGAACGGAa -3' miRNA: 3'- -UAGCUUAGCGGUUCUCCUUCUUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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