Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2099 | 3' | -53.8 | NC_001348.1 | + | 124206 | 0.7 | 0.782841 |
Target: 5'- aGCACGagUGGuucuGCGGCAggguCGGGUUCCa-- -3' miRNA: 3'- -CGUGC--AUUu---CGCCGU----GCCCAAGGcaa -5' |
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2099 | 3' | -53.8 | NC_001348.1 | + | 122315 | 1.08 | 0.004242 |
Target: 5'- gGCACGUAAAGCGGCACGGGUUCCGUUg -3' miRNA: 3'- -CGUGCAUUUCGCCGUGCCCAAGGCAA- -5' |
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2099 | 3' | -53.8 | NC_001348.1 | + | 122129 | 0.68 | 0.869296 |
Target: 5'- gGC-UGUGAgAGCGGCGCGGGc-UCGUUa -3' miRNA: 3'- -CGuGCAUU-UCGCCGUGCCCaaGGCAA- -5' |
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2099 | 3' | -53.8 | NC_001348.1 | + | 107663 | 0.68 | 0.869296 |
Target: 5'- gGC-UGUGAgAGCGGCGCGGGc-UCGUUa -3' miRNA: 3'- -CGuGCAUU-UCGCCGUGCCCaaGGCAA- -5' |
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2099 | 3' | -53.8 | NC_001348.1 | + | 107477 | 1.08 | 0.004242 |
Target: 5'- gGCACGUAAAGCGGCACGGGUUCCGUUg -3' miRNA: 3'- -CGUGCAUUUCGCCGUGCCCAAGGCAA- -5' |
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2099 | 3' | -53.8 | NC_001348.1 | + | 105586 | 0.7 | 0.782841 |
Target: 5'- aGCACGagUGGuucuGCGGCAggguCGGGUUCCa-- -3' miRNA: 3'- -CGUGC--AUUu---CGCCGU----GCCCAAGGcaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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