Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20990 | 5' | -47.6 | NC_004735.1 | + | 12232 | 0.66 | 0.999188 |
Target: 5'- gGCGCAC---CAGUaCGUGGAGAAGu- -3' miRNA: 3'- -CGCGUGuuuGUCGcGCAUUUCUUCuu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 127529 | 0.66 | 0.999188 |
Target: 5'- aGCG-AgGAGCAuGUGCGUGAAGAaauGGAu -3' miRNA: 3'- -CGCgUgUUUGU-CGCGCAUUUCU---UCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 99916 | 0.66 | 0.998495 |
Target: 5'- cGCGUugAuagcucagacAGCAGCGgGUGcuGGAGAc -3' miRNA: 3'- -CGCGugU----------UUGUCGCgCAUuuCUUCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 58683 | 0.67 | 0.998173 |
Target: 5'- aGCGcCACAGGCGGUG-GUAuaucuuccGGGAGAAa -3' miRNA: 3'- -CGC-GUGUUUGUCGCgCAUu-------UCUUCUU- -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 20489 | 0.69 | 0.990518 |
Target: 5'- aGUGCGggUAAGCAGCGC---AAGAAGAu -3' miRNA: 3'- -CGCGU--GUUUGUCGCGcauUUCUUCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 128365 | 0.73 | 0.927283 |
Target: 5'- uUGCGCGA--GGCGCuuGUGAAGAAGAAa -3' miRNA: 3'- cGCGUGUUugUCGCG--CAUUUCUUCUU- -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 38682 | 0.73 | 0.91518 |
Target: 5'- -aGCGCAAGgGGgGCGUAuAGGAAGAc -3' miRNA: 3'- cgCGUGUUUgUCgCGCAU-UUCUUCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 92020 | 0.75 | 0.829423 |
Target: 5'- -aGCAgAAGCAGCaGCGggagGAGGAAGAAg -3' miRNA: 3'- cgCGUgUUUGUCG-CGCa---UUUCUUCUU- -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 127496 | 0.76 | 0.781331 |
Target: 5'- uUGCACAcGCuucuugAGCGCGUAGAGGAGGc -3' miRNA: 3'- cGCGUGUuUG------UCGCGCAUUUCUUCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 39835 | 0.81 | 0.520617 |
Target: 5'- uGCGCAUgcugAAGCAaaauGCGCGUGAGGAAGAu -3' miRNA: 3'- -CGCGUG----UUUGU----CGCGCAUUUCUUCUu -5' |
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20990 | 5' | -47.6 | NC_004735.1 | + | 39969 | 1.09 | 0.014275 |
Target: 5'- uGCGCACAAACAGCGCGUAAAGAAGAAc -3' miRNA: 3'- -CGCGUGUUUGUCGCGCAUUUCUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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