miRNA: 3'- uugGUaACUUUCGaCCCA--CGGGUUUc -5'
miRNA: 3'- -UUGGuAACUU------UCGACCCACGgGUUUC- -5'
miRNA: 3'- -UUGGUAACUUUCGACCCACGGGUUUC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20993 | 3' | -50.8 | NC_004735.1 | + | 84261 | 0.67 | 0.960709 |
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Target: 5'- --aCAcUGGAAGCgGGGUauGCCCAAAa -3' miRNA: 3'- uugGUaACUUUCGaCCCA--CGGGUUUc -5' |
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| 20993 | 3' | -50.8 | NC_004735.1 | + | 111036 | 0.67 | 0.956788 |
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Target: 5'- gGACC-UUGGGuuuaccGGCUGGGUGUaCAGGGa -3' miRNA: 3'- -UUGGuAACUU------UCGACCCACGgGUUUC- -5' |
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| 20993 | 3' | -50.8 | NC_004735.1 | + | 72731 | 1.06 | 0.00851 |
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Target: 5'- aAACCAUUGAAAGCUGGGUGCCCAAAGu -3' miRNA: 3'- -UUGGUAACUUUCGACCCACGGGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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