Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20993 | 5' | -52.2 | NC_004735.1 | + | 21207 | 0.66 | 0.978201 |
Target: 5'- gGCgugUGGUGUGcuu-CCCgCGCGGCg -3' miRNA: 3'- gCGaa-ACUACGCuuguGGGaGUGCCG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 44879 | 0.66 | 0.978201 |
Target: 5'- aCGCggugGAUGCGAAUGuuCgaCGCGGUa -3' miRNA: 3'- -GCGaaa-CUACGCUUGUggGa-GUGCCG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 45407 | 0.67 | 0.963615 |
Target: 5'- cCGCUgaGGUcuucgaGcCGGACACCCUUAcccuCGGCg -3' miRNA: 3'- -GCGAaaCUA------C-GCUUGUGGGAGU----GCCG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 56772 | 0.67 | 0.960032 |
Target: 5'- aCGCUcUGAaaggUGCG-GCGuCUCUCACGcGCa -3' miRNA: 3'- -GCGAaACU----ACGCuUGU-GGGAGUGC-CG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 28981 | 0.68 | 0.922549 |
Target: 5'- gGCUccGGUGgGAGCGCgg-CGCGGCg -3' miRNA: 3'- gCGAaaCUACgCUUGUGggaGUGCCG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 61933 | 0.68 | 0.922549 |
Target: 5'- gGCUaUGA-GCGAACACCUaC-CGGUu -3' miRNA: 3'- gCGAaACUaCGCUUGUGGGaGuGCCG- -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 58349 | 0.71 | 0.828012 |
Target: 5'- gGCUg----GCGAACACCCgcuuugCGCGGg -3' miRNA: 3'- gCGAaacuaCGCUUGUGGGa-----GUGCCg -5' |
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20993 | 5' | -52.2 | NC_004735.1 | + | 72697 | 1.12 | 0.003632 |
Target: 5'- uCGCUUUGAUGCGAACACCCUCACGGCu -3' miRNA: 3'- -GCGAAACUACGCUUGUGGGAGUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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