Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20994 | 3' | -50.7 | NC_004735.1 | + | 126362 | 0.66 | 0.991672 |
Target: 5'- -cGGAUCCUgacuaUCCGGAucCCGGaUUCCa -3' miRNA: 3'- gaCCUAGGA-----AGGUCUu-GGUUaGAGGg -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 72228 | 0.66 | 0.990448 |
Target: 5'- gUGGGUCCagaCGGAAUCAAUaUUCUCa -3' miRNA: 3'- gACCUAGGaagGUCUUGGUUA-GAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 129565 | 0.66 | 0.987578 |
Target: 5'- aUGGAcgUCuCUUCCAccaacaaaacGAACU--UCUCCCg -3' miRNA: 3'- gACCU--AG-GAAGGU----------CUUGGuuAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 1165 | 0.67 | 0.984837 |
Target: 5'- cCUGGAUaCCUcccaccgucauauaaUCCAGAAauCUGAUCguaCCCg -3' miRNA: 3'- -GACCUA-GGA---------------AGGUCUU--GGUUAGa--GGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 116640 | 0.67 | 0.984085 |
Target: 5'- gCUGGAUCCaaCCGGAuucCCGAUugacagcaaucCUCCg -3' miRNA: 3'- -GACCUAGGaaGGUCUu--GGUUA-----------GAGGg -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 45919 | 0.67 | 0.984085 |
Target: 5'- gCUGGAaaaUCUUaCCAGAAgaCGAUC-CCCa -3' miRNA: 3'- -GACCU---AGGAaGGUCUUg-GUUAGaGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 16191 | 0.67 | 0.979892 |
Target: 5'- -gGGGucUCCUUCCAGAuCUucgCUCCa -3' miRNA: 3'- gaCCU--AGGAAGGUCUuGGuuaGAGGg -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 113820 | 0.67 | 0.97751 |
Target: 5'- gCUGGAagaagaacuUCUuaacagaaaaaUUCCGGuAGgCAAUCUCCCg -3' miRNA: 3'- -GACCU---------AGG-----------AAGGUC-UUgGUUAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 48593 | 0.67 | 0.977261 |
Target: 5'- -cGGGU-CUUCCAGAgaaaucuuucuACCGAaaagcaaaugcacUCUCCCc -3' miRNA: 3'- gaCCUAgGAAGGUCU-----------UGGUU-------------AGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 107617 | 0.68 | 0.972135 |
Target: 5'- uUGGAUCaaUCCAGAu--GGUUUCCCu -3' miRNA: 3'- gACCUAGgaAGGUCUuggUUAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 89832 | 0.69 | 0.950578 |
Target: 5'- uCUGGAUUCc-CCaAGcACCAGaUCUCCCg -3' miRNA: 3'- -GACCUAGGaaGG-UCuUGGUU-AGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 58642 | 0.73 | 0.789685 |
Target: 5'- gUGGAUUCcgCCGGAaaugaaauggugguuACCGGUCUUCCa -3' miRNA: 3'- gACCUAGGaaGGUCU---------------UGGUUAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 39170 | 0.78 | 0.525335 |
Target: 5'- -cGGAUCgagCUcCCAGAacaGCCGGUCUCCCg -3' miRNA: 3'- gaCCUAG---GAaGGUCU---UGGUUAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 110185 | 0.79 | 0.49486 |
Target: 5'- uUGGAUUCUUUUAcGAUCAAUCUCCCg -3' miRNA: 3'- gACCUAGGAAGGUcUUGGUUAGAGGG- -5' |
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20994 | 3' | -50.7 | NC_004735.1 | + | 79955 | 1.14 | 0.00409 |
Target: 5'- gCUGGAUCCUUCCAGAACCAAUCUCCCg -3' miRNA: 3'- -GACCUAGGAAGGUCUUGGUUAGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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