Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20994 | 5' | -48.1 | NC_004735.1 | + | 54634 | 0.66 | 0.999291 |
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Target: 5'- --uGGAAGccAACCCUGA--AUGGGAa -3' miRNA: 3'- ccuUCUUCaaUUGGGGCUugUACCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 44200 | 0.66 | 0.998926 |
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Target: 5'- gGGAuuGGAuuuGUUAccagaaucCCCUGGAUGUGGGAu -3' miRNA: 3'- -CCU--UCUu--CAAUu-------GGGGCUUGUACCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 57595 | 0.68 | 0.995259 |
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Target: 5'- cGGAGGAacaucuuccggguaGGUg--UCCCGAAUAUaGGGAa -3' miRNA: 3'- -CCUUCU--------------UCAauuGGGGCUUGUA-CCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 38071 | 0.68 | 0.993841 |
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Target: 5'- gGGGAGAagacguGGUUGucguauauGCCCCGcuuGGCAuuuUGGGAa -3' miRNA: 3'- -CCUUCU------UCAAU--------UGGGGC---UUGU---ACCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 128308 | 0.68 | 0.993841 |
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Target: 5'- cGggGAuGgaacAGCCCUuuGAGCGUGGGGa -3' miRNA: 3'- cCuuCUuCaa--UUGGGG--CUUGUACCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 95482 | 0.69 | 0.991756 |
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Target: 5'- uGGAGAAGUU--CCCCGugauCcgGGGGa -3' miRNA: 3'- cCUUCUUCAAuuGGGGCuu--GuaCCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 109143 | 0.75 | 0.861971 |
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Target: 5'- cGGAAGggGUUAACCCUaAACucuGGAa -3' miRNA: 3'- -CCUUCuuCAAUUGGGGcUUGuacCCU- -5' |
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| 20994 | 5' | -48.1 | NC_004735.1 | + | 79920 | 1.13 | 0.009183 |
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Target: 5'- uGGAAGAAGUUAACCCCGAACAUGGGAg -3' miRNA: 3'- -CCUUCUUCAAUUGGGGCUUGUACCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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