Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20997 | 5' | -48.5 | NC_004735.1 | + | 102453 | 0.66 | 0.999066 |
Target: 5'- gCGUCGucAAGUUUUucGAcAGCAgCCCCg -3' miRNA: 3'- -GCAGCucUUCGAAAu-CU-UUGUgGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 129490 | 0.66 | 0.999066 |
Target: 5'- ---aGAGAGGgUgugggaaaGGAAACACCCUCu -3' miRNA: 3'- gcagCUCUUCgAaa------UCUUUGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 45696 | 0.66 | 0.998857 |
Target: 5'- -uUCGGGAcGGUUgac--AACGCCCCCg -3' miRNA: 3'- gcAGCUCU-UCGAaaucuUUGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 371 | 0.67 | 0.997619 |
Target: 5'- cCGUCGGGcuGCUgcuccuccaccGACACCCUCu -3' miRNA: 3'- -GCAGCUCuuCGAaaucu------UUGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 110401 | 0.67 | 0.997576 |
Target: 5'- --aCGAGAAGCUUcAGAGGCugCa-- -3' miRNA: 3'- gcaGCUCUUCGAAaUCUUUGugGggg -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 29797 | 0.67 | 0.997576 |
Target: 5'- -uUCGAGggGCUUUuuuGAc-CGCCUCUu -3' miRNA: 3'- gcAGCUCuuCGAAAu--CUuuGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 39088 | 0.67 | 0.996582 |
Target: 5'- gCGUCGGGGuugGGgUUUGGA---ACCUCCg -3' miRNA: 3'- -GCAGCUCU---UCgAAAUCUuugUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 62393 | 0.7 | 0.976788 |
Target: 5'- --cUGAGAcGCUUUccGGAGAUACCaCCCu -3' miRNA: 3'- gcaGCUCUuCGAAA--UCUUUGUGG-GGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 24336 | 0.74 | 0.891078 |
Target: 5'- gCGggGAGAAGUgcugAGAccGCACCCCCg -3' miRNA: 3'- -GCagCUCUUCGaaa-UCUu-UGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 110903 | 0.74 | 0.883854 |
Target: 5'- gGUCcAGAAGU-UUGGGAAUAUCCCCu -3' miRNA: 3'- gCAGcUCUUCGaAAUCUUUGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 9351 | 0.76 | 0.799042 |
Target: 5'- uGUUGuGAAGCUgauGAccCACCCCCg -3' miRNA: 3'- gCAGCuCUUCGAaauCUuuGUGGGGG- -5' |
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20997 | 5' | -48.5 | NC_004735.1 | + | 123070 | 1.14 | 0.006757 |
Target: 5'- uCGUCGAGAAGCUUUAGAAACACCCCCa -3' miRNA: 3'- -GCAGCUCUUCGAAAUCUUUGUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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