Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21000 | 3' | -52.4 | NC_004735.1 | + | 124017 | 0.66 | 0.979066 |
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Target: 5'- aUGAUGAAGgGCGGUaUCaUACCcuGCuGGa -3' miRNA: 3'- gACUACUUCgUGCCAaAG-GUGG--CG-CC- -5' |
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| 21000 | 3' | -52.4 | NC_004735.1 | + | 53671 | 0.66 | 0.96822 |
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Target: 5'- uCUGAUGAAGUGCauacgCCACUGCa- -3' miRNA: 3'- -GACUACUUCGUGccaaaGGUGGCGcc -5' |
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| 21000 | 3' | -52.4 | NC_004735.1 | + | 74002 | 0.69 | 0.907366 |
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Target: 5'- -cGAUuacuGCuACGGUUUCCGCCGCc- -3' miRNA: 3'- gaCUAcuu-CG-UGCCAAAGGUGGCGcc -5' |
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| 21000 | 3' | -52.4 | NC_004735.1 | + | 101597 | 0.7 | 0.880234 |
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Target: 5'- -aGAuUGggGUA-GGUUUCCAgCGUGGa -3' miRNA: 3'- gaCU-ACuuCGUgCCAAAGGUgGCGCC- -5' |
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| 21000 | 3' | -52.4 | NC_004735.1 | + | 322 | 0.75 | 0.646222 |
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Target: 5'- uCUGAUaGGAGCGUGGUUUCUGCUGCuGGu -3' miRNA: 3'- -GACUA-CUUCGUGCCAAAGGUGGCG-CC- -5' |
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| 21000 | 3' | -52.4 | NC_004735.1 | + | 129 | 1.1 | 0.005106 |
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Target: 5'- uCUGAUGAAGCACGGUUUCCACCGCGGc -3' miRNA: 3'- -GACUACUUCGUGCCAAAGGUGGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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