Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21001 | 3' | -46.2 | NC_004735.1 | + | 1924 | 0.66 | 0.999887 |
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Target: 5'- aAAUUCUAaAAUCCCaAUGGGuaAUAUGCg -3' miRNA: 3'- -UUAAGAUaUUGGGGaUGUCC--UAUGCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 92085 | 0.66 | 0.999807 |
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Target: 5'- --aUCUu---CCgCCUGCAGGAUAuUGCc -3' miRNA: 3'- uuaAGAuauuGG-GGAUGUCCUAU-GCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 25986 | 0.67 | 0.999196 |
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Target: 5'- cAUUCUGUAugCCgU-CAGGGUG-GCa -3' miRNA: 3'- uUAAGAUAUugGGgAuGUCCUAUgCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 55392 | 0.67 | 0.999196 |
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Target: 5'- --gUCggGUAAUauUCCU-CAGGAUACGCu -3' miRNA: 3'- uuaAGa-UAUUG--GGGAuGUCCUAUGCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 82587 | 0.68 | 0.999005 |
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Target: 5'- -------aGAUCCCUACAGGAUAUu- -3' miRNA: 3'- uuaagauaUUGGGGAUGUCCUAUGcg -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 1808 | 0.71 | 0.983769 |
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Target: 5'- -uUUCUuUAAUCUCcAUAGGGUACGCu -3' miRNA: 3'- uuAAGAuAUUGGGGaUGUCCUAUGCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 1888 | 0.71 | 0.983769 |
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Target: 5'- -uUUCUuUAAUCUCcAUAGGGUACGCu -3' miRNA: 3'- uuAAGAuAUUGGGGaUGUCCUAUGCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 95407 | 0.74 | 0.952152 |
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Target: 5'- uAGUUCUuu-ACCCCUACGGGAccccggaGCa -3' miRNA: 3'- -UUAAGAuauUGGGGAUGUCCUaug----CG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 84064 | 0.78 | 0.808717 |
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Target: 5'- -uUUUUGUcgAACCCCuUGCGGGGUACGUu -3' miRNA: 3'- uuAAGAUA--UUGGGG-AUGUCCUAUGCG- -5' |
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| 21001 | 3' | -46.2 | NC_004735.1 | + | 1727 | 1.11 | 0.015605 |
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Target: 5'- aAAUUCUAUAACCCCUACAGGAUACGCu -3' miRNA: 3'- -UUAAGAUAUUGGGGAUGUCCUAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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