Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21003 | 3' | -47.9 | NC_004735.1 | + | 114652 | 0.66 | 0.999008 |
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Target: 5'- --uUUCa-GAUAuGCCGGGGGAAUa- -3' miRNA: 3'- uguAAGacCUAUuUGGCCCCCUUAag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 32273 | 0.66 | 0.998988 |
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Target: 5'- -gGUUCUGGAguaauagUAGGgcuUCGGGGGAAa-- -3' miRNA: 3'- ugUAAGACCU-------AUUU---GGCCCCCUUaag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 69771 | 0.67 | 0.997836 |
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Target: 5'- ---aUCUGG-UAAcuucAUCGGGGGAcUUCa -3' miRNA: 3'- uguaAGACCuAUU----UGGCCCCCUuAAG- -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 123152 | 0.67 | 0.997836 |
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Target: 5'- aGCA-UUUGGAgccaguUCGGGGGGAUUa -3' miRNA: 3'- -UGUaAGACCUauuu--GGCCCCCUUAAg -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 107207 | 0.67 | 0.99691 |
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Target: 5'- ---aUCUGGu--AGCUGGGGGAu--- -3' miRNA: 3'- uguaAGACCuauUUGGCCCCCUuaag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 125528 | 0.68 | 0.994069 |
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Target: 5'- ------gGuGAUGAACCGGGGGuuUUCc -3' miRNA: 3'- uguaagaC-CUAUUUGGCCCCCuuAAG- -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 116688 | 0.72 | 0.940299 |
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Target: 5'- uCAUUCgcuugGGGuUGGAUCGGGGGGAUa- -3' miRNA: 3'- uGUAAGa----CCU-AUUUGGCCCCCUUAag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 68631 | 0.72 | 0.935127 |
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Target: 5'- uUAUUCUGGuaauAGCCGGGGGcAUa- -3' miRNA: 3'- uGUAAGACCuau-UUGGCCCCCuUAag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 114028 | 0.74 | 0.868393 |
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Target: 5'- aACG-UCUGGGauuuuGACCGGGGGAAa-- -3' miRNA: 3'- -UGUaAGACCUau---UUGGCCCCCUUaag -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 112368 | 0.83 | 0.468098 |
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Target: 5'- cGCGUUCUGGAagAAACgGaGGGGGAUUCu -3' miRNA: 3'- -UGUAAGACCUa-UUUGgC-CCCCUUAAG- -5' |
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| 21003 | 3' | -47.9 | NC_004735.1 | + | 11889 | 1.11 | 0.010108 |
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Target: 5'- cACAUUCUGGAUAAACCGGGGGAAUUCg -3' miRNA: 3'- -UGUAAGACCUAUUUGGCCCCCUUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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