Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21003 | 5' | -41.7 | NC_004735.1 | + | 46002 | 0.67 | 1 |
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Target: 5'- uGAAAUUCCCCUUgUUUUGUacaaccagUUCAGAAa -3' miRNA: 3'- cUUUUAGGGGGAA-AAAACA--------GAGUUUU- -5' |
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| 21003 | 5' | -41.7 | NC_004735.1 | + | 77113 | 0.66 | 1 |
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Target: 5'- -----aCCCCUUaUUUUGUCUCu--- -3' miRNA: 3'- cuuuuaGGGGGAaAAAACAGAGuuuu -5' |
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| 21003 | 5' | -41.7 | NC_004735.1 | + | 12839 | 0.66 | 1 |
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Target: 5'- cGAAAAUCCCCUUUacc-GcCUCGAu- -3' miRNA: 3'- -CUUUUAGGGGGAAaaaaCaGAGUUuu -5' |
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| 21003 | 5' | -41.7 | NC_004735.1 | + | 40905 | 0.69 | 0.999997 |
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Target: 5'- uGAGcgCCCCUUUUUUUaUCUUAAAAa -3' miRNA: 3'- cUUUuaGGGGGAAAAAAcAGAGUUUU- -5' |
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| 21003 | 5' | -41.7 | NC_004735.1 | + | 128993 | 0.69 | 0.999993 |
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Target: 5'- uGAAGAUCCCCCacacaUUUUUUGauauUCUCu--- -3' miRNA: 3'- -CUUUUAGGGGG-----AAAAAAC----AGAGuuuu -5' |
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| 21003 | 5' | -41.7 | NC_004735.1 | + | 11928 | 1.08 | 0.066077 |
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Target: 5'- cGAAAAUCCCCCUUUUUUGUCUCAAAAc -3' miRNA: 3'- -CUUUUAGGGGGAAAAAACAGAGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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