Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21004 | 5' | -49.1 | NC_004735.1 | + | 125722 | 0.66 | 0.995874 |
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Target: 5'- cACCaUCGCGguuGUCGUuacccGGCGAAAGCc -3' miRNA: 3'- uUGGgAGCGUau-UAGCG-----CCGUUUUUG- -5' |
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| 21004 | 5' | -49.1 | NC_004735.1 | + | 78063 | 0.66 | 0.994317 |
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Target: 5'- uAGCCU--GCAUGGUgGUGGCAAGAu- -3' miRNA: 3'- -UUGGGagCGUAUUAgCGCCGUUUUug -5' |
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| 21004 | 5' | -49.1 | NC_004735.1 | + | 127239 | 0.68 | 0.980661 |
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Target: 5'- cACCCggcuuuccCGCAUAcggCGCGGCGucGGACa -3' miRNA: 3'- uUGGGa-------GCGUAUua-GCGCCGUu-UUUG- -5' |
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| 21004 | 5' | -49.1 | NC_004735.1 | + | 62699 | 0.68 | 0.975659 |
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Target: 5'- aAACCUgaGCGUAGUCGaCGGCGAu--- -3' miRNA: 3'- -UUGGGagCGUAUUAGC-GCCGUUuuug -5' |
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| 21004 | 5' | -49.1 | NC_004735.1 | + | 102505 | 0.77 | 0.650877 |
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Target: 5'- cGCCUgaGCaAUGAUCGUGGCAAAAACg -3' miRNA: 3'- uUGGGagCG-UAUUAGCGCCGUUUUUG- -5' |
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| 21004 | 5' | -49.1 | NC_004735.1 | + | 13648 | 1.09 | 0.009101 |
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Target: 5'- gAACCCUCGCAUAAUCGCGGCAAAAACa -3' miRNA: 3'- -UUGGGAGCGUAUUAGCGCCGUUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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