Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21006 | 3' | -51.7 | NC_004735.1 | + | 58492 | 0.66 | 0.984919 |
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Target: 5'- -gUGCCGAaaUCUCAgGUGucGCUUCCGGu -3' miRNA: 3'- agAUGGCUa-AGGGU-CGCu-UGAAGGCC- -5' |
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| 21006 | 3' | -51.7 | NC_004735.1 | + | 119026 | 0.66 | 0.983027 |
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Target: 5'- --gACaCGuacAUUCCCAGCGAGgUUCCu- -3' miRNA: 3'- agaUG-GC---UAAGGGUCGCUUgAAGGcc -5' |
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| 21006 | 3' | -51.7 | NC_004735.1 | + | 21075 | 0.67 | 0.976274 |
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Target: 5'- --cGCCGuugauacUUCCCAcCGAAgUUCCGGc -3' miRNA: 3'- agaUGGCu------AAGGGUcGCUUgAAGGCC- -5' |
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| 21006 | 3' | -51.7 | NC_004735.1 | + | 39792 | 0.69 | 0.929688 |
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Target: 5'- cCUuUCGAUUCaCCAGCGAcuccaacucucGCUUgCGGa -3' miRNA: 3'- aGAuGGCUAAG-GGUCGCU-----------UGAAgGCC- -5' |
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| 21006 | 3' | -51.7 | NC_004735.1 | + | 14605 | 0.71 | 0.848005 |
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Target: 5'- aUUUGCCGGUUCCgGcGCGAACcagacgcgUCUGGu -3' miRNA: 3'- -AGAUGGCUAAGGgU-CGCUUGa-------AGGCC- -5' |
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| 21006 | 3' | -51.7 | NC_004735.1 | + | 27314 | 1.11 | 0.00506 |
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Target: 5'- aUCUACCGAUUCCCAGCGAACUUCCGGu -3' miRNA: 3'- -AGAUGGCUAAGGGUCGCUUGAAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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