Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21006 | 5' | -56.2 | NC_004735.1 | + | 298 | 0.66 | 0.888407 |
Target: 5'- -gCUGGUGAuUUuuGCCGCGgUGGAa -3' miRNA: 3'- ggGGCUACUuAAggCGGCGCaGCCUa -5' |
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21006 | 5' | -56.2 | NC_004735.1 | + | 59455 | 0.68 | 0.782887 |
Target: 5'- aCCCCGGUGGuccauGUUCCGCCugauauguUGUgGGAg -3' miRNA: 3'- -GGGGCUACU-----UAAGGCGGc-------GCAgCCUa -5' |
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21006 | 5' | -56.2 | NC_004735.1 | + | 27350 | 1.07 | 0.00354 |
Target: 5'- cCCCCGAUGAAUUCCGCCGCGUCGGAUc -3' miRNA: 3'- -GGGGCUACUUAAGGCGGCGCAGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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