Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21007 | 5' | -55.8 | NC_004735.1 | + | 19662 | 0.66 | 0.897895 |
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Target: 5'- aCGGCgUGCCGGgauugacggaaguuaAGCGaUCGCAuUCGCUg -3' miRNA: 3'- -GUCGgAUGGCC---------------UCGUaAGCGU-GGUGG- -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 62095 | 0.66 | 0.89525 |
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Target: 5'- cCAGCUUaaaACCGGuaGGUGUUCGCuCauaGCCg -3' miRNA: 3'- -GUCGGA---UGGCC--UCGUAAGCGuGg--UGG- -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 26213 | 0.66 | 0.888474 |
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Target: 5'- gUAGCCUcCCGaAGCGaa-GCGCCGCa -3' miRNA: 3'- -GUCGGAuGGCcUCGUaagCGUGGUGg -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 13409 | 0.66 | 0.881465 |
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Target: 5'- -uGCUUGCCGGGGUuucuccgggUUGCcacCCACCc -3' miRNA: 3'- guCGGAUGGCCUCGua-------AGCGu--GGUGG- -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 3017 | 0.66 | 0.866013 |
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Target: 5'- uCAGCUUGCCGGGGU--UCGCGgaaaacuUCAUg -3' miRNA: 3'- -GUCGGAUGGCCUCGuaAGCGU-------GGUGg -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 101677 | 0.7 | 0.702926 |
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Target: 5'- cCAGCaugcCCGGAGCAgcaaUCGUACCgacggcACCa -3' miRNA: 3'- -GUCGgau-GGCCUCGUa---AGCGUGG------UGG- -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 87165 | 0.73 | 0.498845 |
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Target: 5'- gCAGCUUACCGGAaaacuucucgauGCGUUCGaCGCgCugCa -3' miRNA: 3'- -GUCGGAUGGCCU------------CGUAAGC-GUG-GugG- -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 9421 | 0.8 | 0.205621 |
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Target: 5'- cCAGCCcuuggcgACCGGGGCGggUGCACCACa -3' miRNA: 3'- -GUCGGa------UGGCCUCGUaaGCGUGGUGg -5' |
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| 21007 | 5' | -55.8 | NC_004735.1 | + | 28938 | 0.99 | 0.011225 |
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Target: 5'- gCAG-CUACCGGAGCAUUCGCACCACCg -3' miRNA: 3'- -GUCgGAUGGCCUCGUAAGCGUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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