Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2101 | 5' | -49.8 | NC_001348.1 | + | 102609 | 0.7 | 0.949298 |
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Target: 5'- uGGCGGGGGu---GGUGAAGGCGAGGg -3' miRNA: 3'- gCUGCUCCUucucCUACUUCUGCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 51977 | 0.67 | 0.990335 |
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Target: 5'- gGugGAGGAuAGcGGGAUGAccgaagccaaGGAUGggGg -3' miRNA: 3'- gCugCUCCU-UC-UCCUACU----------UCUGCuuU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 48802 | 0.83 | 0.35542 |
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Target: 5'- uGuuGAGGAAGAgauGGAUGAAGACGAGAg -3' miRNA: 3'- gCugCUCCUUCU---CCUACUUCUGCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 14204 | 0.88 | 0.184632 |
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Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGAu- -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUuu -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 14156 | 0.88 | 0.189594 |
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Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGAc- -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUuu -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 14109 | 0.8 | 0.464979 |
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Target: 5'- gGAgGAGGGAGAGGcgGAGGAgGGAGa -3' miRNA: 3'- gCUgCUCCUUCUCCuaCUUCUgCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 14078 | 0.9 | 0.141028 |
|
Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGGAGa -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 14030 | 0.9 | 0.141028 |
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Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGGAGa -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 13982 | 0.9 | 0.141028 |
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Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGGAGa -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 13935 | 0.9 | 0.141028 |
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Target: 5'- aCGACGAGGGAGAGGcgGAGGAgGGAGa -3' miRNA: 3'- -GCUGCUCCUUCUCCuaCUUCUgCUUU- -5' |
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| 2101 | 5' | -49.8 | NC_001348.1 | + | 13459 | 0.7 | 0.949298 |
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Target: 5'- cCGGC-AGGAucucGGAUGggGACGGAGg -3' miRNA: 3'- -GCUGcUCCUucu-CCUACuuCUGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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