Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21010 | 5' | -47.8 | NC_004735.1 | + | 457 | 0.67 | 0.997821 |
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Target: 5'- ----aAACGGCAugAAGuAGGGcgcgccuccaaucaUGGCGg -3' miRNA: 3'- gcaaaUUGCCGUugUUU-UCCC--------------ACCGC- -5' |
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| 21010 | 5' | -47.8 | NC_004735.1 | + | 44822 | 0.68 | 0.996505 |
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Target: 5'- uCGUUgcacGCGGCGGCAAAcgcucgcauGGUGGUGu -3' miRNA: 3'- -GCAAau--UGCCGUUGUUUuc-------CCACCGC- -5' |
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| 21010 | 5' | -47.8 | NC_004735.1 | + | 40586 | 0.7 | 0.985769 |
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Target: 5'- cCGUUaAACGugaaaucuaucuugaGCcGCAAAaaGGGGUGGCGg -3' miRNA: 3'- -GCAAaUUGC---------------CGuUGUUU--UCCCACCGC- -5' |
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| 21010 | 5' | -47.8 | NC_004735.1 | + | 15346 | 0.71 | 0.976032 |
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Target: 5'- uCGggaagGGCGG-AACAugGGGGUGGUGg -3' miRNA: 3'- -GCaaa--UUGCCgUUGUuuUCCCACCGC- -5' |
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| 21010 | 5' | -47.8 | NC_004735.1 | + | 62159 | 0.73 | 0.942465 |
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Target: 5'- cCGUUgaUGACGGUAACGGAGcGGGUuGaGCGu -3' miRNA: 3'- -GCAA--AUUGCCGUUGUUUU-CCCA-C-CGC- -5' |
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| 21010 | 5' | -47.8 | NC_004735.1 | + | 40447 | 1.11 | 0.012696 |
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Target: 5'- aCGUUUAACGGCAACAAAAGGGUGGCGu -3' miRNA: 3'- -GCAAAUUGCCGUUGUUUUCCCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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