Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21012 | 3' | -54.5 | NC_004735.1 | + | 10176 | 0.66 | 0.929179 |
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Target: 5'- aCUCCCUCUA-CGAAAuGGauUAUGAGGu -3' miRNA: 3'- cGAGGGAGGUcGCUUUuCC--GUGCUCC- -5' |
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| 21012 | 3' | -54.5 | NC_004735.1 | + | 109477 | 0.67 | 0.893031 |
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Target: 5'- uUUCUUUUCAGCGGuaaccgGAAGuGCAUGGGGa -3' miRNA: 3'- cGAGGGAGGUCGCU------UUUC-CGUGCUCC- -5' |
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| 21012 | 3' | -54.5 | NC_004735.1 | + | 38738 | 0.67 | 0.886167 |
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Target: 5'- aGC-CCCgCCGGCGGAGc-GCACGGGc -3' miRNA: 3'- -CGaGGGaGGUCGCUUUucCGUGCUCc -5' |
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| 21012 | 3' | -54.5 | NC_004735.1 | + | 61278 | 0.68 | 0.864208 |
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Target: 5'- uGCUUCUUCaggaacaAGCGAAAuGGUggGAGGa -3' miRNA: 3'- -CGAGGGAGg------UCGCUUUuCCGugCUCC- -5' |
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| 21012 | 3' | -54.5 | NC_004735.1 | + | 38592 | 0.74 | 0.565035 |
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Target: 5'- cGCUCCg-CCGGCGggGcuuuaAGGUACGGGu -3' miRNA: 3'- -CGAGGgaGGUCGCuuU-----UCCGUGCUCc -5' |
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| 21012 | 3' | -54.5 | NC_004735.1 | + | 45115 | 1.14 | 0.001722 |
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Target: 5'- cGCUCCCUCCAGCGAAAAGGCACGAGGg -3' miRNA: 3'- -CGAGGGAGGUCGCUUUUCCGUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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