Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21013 | 5' | -48.7 | NC_004735.1 | + | 95107 | 0.66 | 0.998148 |
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Target: 5'- --cCUCaAAACGGaGggUUGg-UGUCGCa -3' miRNA: 3'- auaGAG-UUUGCC-CuuGACgaACAGCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 126664 | 0.66 | 0.997518 |
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Target: 5'- gGUCUCGAGCggugcgguguacuauGuGGAugUGCcgGUUGCg -3' miRNA: 3'- aUAGAGUUUG---------------C-CCUugACGaaCAGCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 104320 | 0.66 | 0.997336 |
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Target: 5'- aUAUCUC---CGGGGcuGCUGCUUaUUGCc -3' miRNA: 3'- -AUAGAGuuuGCCCU--UGACGAAcAGCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 16318 | 0.67 | 0.99297 |
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Target: 5'- gGUCUUGGuCGGGGAgaGCUUGgaaUCGUu -3' miRNA: 3'- aUAGAGUUuGCCCUUgaCGAAC---AGCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 96265 | 0.68 | 0.98926 |
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Target: 5'- gAUUUCGggUGGGAAUUuuUUGUCuGCa -3' miRNA: 3'- aUAGAGUuuGCCCUUGAcgAACAG-CG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 58603 | 0.68 | 0.98604 |
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Target: 5'- gAUCUguGGCGGGAGCUuaguaagaaaGCggaUGUgGCg -3' miRNA: 3'- aUAGAguUUGCCCUUGA----------CGa--ACAgCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 26544 | 0.73 | 0.898241 |
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Target: 5'- cAUCUCAAAgGGGAuGgUGCUU-UCGCa -3' miRNA: 3'- aUAGAGUUUgCCCU-UgACGAAcAGCG- -5' |
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| 21013 | 5' | -48.7 | NC_004735.1 | + | 48668 | 1.11 | 0.008167 |
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Target: 5'- aUAUCUCAAACGGGAACUGCUUGUCGCu -3' miRNA: 3'- -AUAGAGUUUGCCCUUGACGAACAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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