miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21015 3' -52.9 NC_004735.1 + 9521 0.66 0.963387
Target:  5'- uCCGG-CUcCUCUGUACA-ACAUCCCc -3'
miRNA:   3'- -GGCUaGGuGAGACGUGUuUGUGGGGu -5'
21015 3' -52.9 NC_004735.1 + 44622 0.67 0.953585
Target:  5'- gCUGGUCCuCUCUaaggagcagcgagaaGCACuuGACACCaCCAu -3'
miRNA:   3'- -GGCUAGGuGAGA---------------CGUGu-UUGUGG-GGU- -5'
21015 3' -52.9 NC_004735.1 + 72977 0.67 0.938295
Target:  5'- aCGAugUUCGCUUUGCuACuuccCACCCCAa -3'
miRNA:   3'- gGCU--AGGUGAGACG-UGuuu-GUGGGGU- -5'
21015 3' -52.9 NC_004735.1 + 93221 0.68 0.90423
Target:  5'- uCCGGUacCCAUuucgUCUGCAaugaGAGCACCCgGa -3'
miRNA:   3'- -GGCUA--GGUG----AGACGUg---UUUGUGGGgU- -5'
21015 3' -52.9 NC_004735.1 + 114662 0.7 0.851754
Target:  5'- gCCGGgggaauauaaaCAUcgUCgUGCACAAACACCCCGg -3'
miRNA:   3'- -GGCUag---------GUG--AG-ACGUGUUUGUGGGGU- -5'
21015 3' -52.9 NC_004735.1 + 23147 0.7 0.836838
Target:  5'- -aGAUCaCGCUaaGCGCAAGCguauACCCCGa -3'
miRNA:   3'- ggCUAG-GUGAgaCGUGUUUG----UGGGGU- -5'
21015 3' -52.9 NC_004735.1 + 57775 0.7 0.819518
Target:  5'- cUCGAaCCACUCUGCAUGAu--CCUCAu -3'
miRNA:   3'- -GGCUaGGUGAGACGUGUUuguGGGGU- -5'
21015 3' -52.9 NC_004735.1 + 11379 0.7 0.819518
Target:  5'- uUCGAUCUACUCccauagGCGCAaaagucagaAACACCCg- -3'
miRNA:   3'- -GGCUAGGUGAGa-----CGUGU---------UUGUGGGgu -5'
21015 3' -52.9 NC_004735.1 + 6549 0.75 0.566992
Target:  5'- uCgGAaCCGCUCUGCAUGGgaguuuuuacguGCGCCCCAu -3'
miRNA:   3'- -GgCUaGGUGAGACGUGUU------------UGUGGGGU- -5'
21015 3' -52.9 NC_004735.1 + 73312 1.12 0.003331
Target:  5'- aCCGAUCCACUCUGCACAAACACCCCAg -3'
miRNA:   3'- -GGCUAGGUGAGACGUGUUUGUGGGGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.