Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21018 | 5' | -50.3 | NC_004735.1 | + | 34097 | 0.66 | 0.990898 |
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Target: 5'- gGCGGGGGuuguuuccucACCGAau--CCGCCCGu-- -3' miRNA: 3'- -UGCCCUU----------UGGUUauuuGGCGGGCuua -5' |
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| 21018 | 5' | -50.3 | NC_004735.1 | + | 127384 | 0.66 | 0.986457 |
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Target: 5'- uGCGGGaAAGCCGGgugAAGaaGCCCGu-- -3' miRNA: 3'- -UGCCC-UUUGGUUa--UUUggCGGGCuua -5' |
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| 21018 | 5' | -50.3 | NC_004735.1 | + | 21991 | 0.67 | 0.980513 |
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Target: 5'- gACGGGAAACCAuucauauUAAACa-CCUGAu- -3' miRNA: 3'- -UGCCCUUUGGUu------AUUUGgcGGGCUua -5' |
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| 21018 | 5' | -50.3 | NC_004735.1 | + | 2889 | 0.68 | 0.969185 |
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Target: 5'- uACGGGAAGugugcucaauguuCCAAUGAACCGCagucaauUCGAc- -3' miRNA: 3'- -UGCCCUUU-------------GGUUAUUUGGCG-------GGCUua -5' |
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| 21018 | 5' | -50.3 | NC_004735.1 | + | 51863 | 0.68 | 0.963119 |
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Target: 5'- aAUGGGAAACUAAUAguAGCCGaaucaaCUGAAc -3' miRNA: 3'- -UGCCCUUUGGUUAU--UUGGCg-----GGCUUa -5' |
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| 21018 | 5' | -50.3 | NC_004735.1 | + | 84619 | 1.06 | 0.011477 |
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Target: 5'- aACGGGAAACCAAUAAACCGCCCGAAUa -3' miRNA: 3'- -UGCCCUUUGGUUAUUUGGCGGGCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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